Difference between revisions of "Part:BBa K2559000"

Revision as of 03:09, 13 October 2018

Bacterial cellulose synthase Z (BcsZ) The BBa_K2559000 is an endoglucanase (Bcs Z) involved in the process of cellulose hydrolysis, and its roles in cellulose biosynthesis have long remained obscure.

Usage and Biology

Bacterial cellulose synthase Z (BcsZ) from several bacteria have been shown to possess an endo-β-1,4-glucanase (cellulase) activity, and E. coli protein was even crystallized in a complex with the substrate cellopentaose(Figure 2), however, the roles in cellulose biosynthesis have long remained obscure. We suspect that the BcsZ and BlgX may help the cell form the correct cellulose configuration through selective digestion. We introduced the Acetobacter xylinum bacterial cellulose synthase gene (including BcsZ, H, A, B, C, D, LX) into the model organism cyanobacteria (Synechocystis),and we obtain the transgenic cyanobacteria(Figure 3).

Transcriptional activity of PSY

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 252

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 ===Usage and Biology===
-Bacterial cellulose synthase Z (BcsZ) from several bacteria have been shown to possess an endo-β-1,4-glucanase (cellulase) activity, and E. coli protein was even crystallized in a complex with the substrate cellopentaose, however, and the roles in cellulose biosynthesis have long remained obscure. We suspect that the BcsZ and BlgX may help the cell form the correct cellulose configuration through selective digestion.We introduced the Acetobacter xylinum bacterial cellulose synthase gene (including BcsZ, H, A, B, C, D, LX) into the model organism Synechocystis, the part is facilitate the in-depth research  for other teams.
+Bacterial cellulose synthase Z (BcsZ) from several bacteria have been shown to possess an endo-β-1,4-glucanase (cellulase) activity, and E. coli protein was even crystallized in a complex with the substrate cellopentaose(Figure 2), however, the roles in cellulose biosynthesis have long remained obscure. We suspect that the BcsZ and BlgX may help the cell form the correct cellulose configuration through selective digestion.
+We introduced the Acetobacter xylinum bacterial cellulose synthase gene (including BcsZ, H, A, B, C, D, LX) into the model organism cyanobacteria (Synechocystis),and we obtain the transgenic cyanobacteria(Figure 3).
 ===Transcriptional activity of ''PSY''===