Difference between revisions of "Part:BBa K2680116"
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<partinfo>BBa_K2680116 short</partinfo>
 
<partinfo>BBa_K2680116 short</partinfo>
  
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3G-compatible pLac/ara-1 dual input promoter. Repressed by LacI and activated by arabinose/AraC. Intended for use in oscillator creation. Sequence originally from Lutz and Bujard 1997; later used for the creation of oscillator from Stricker et al 2008. Note that the upstream LacO site is required for functional repression via LacI (tetrameter) [See Stricker et al 2008 supplement].
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3G-compatible pLac/ara-1 dual input promoter. Flanked by prefix sticky end A and suffix sticky end B. Repressed by LacI and activated by arabinose/AraC. Intended for use in oscillator creation. Sequence originally from Lutz and Bujard 1997; later used for the creation of oscillator from Stricker et al 2008. Note that the upstream LacO site is required for functional repression via LacI (tetrameter) [See Stricker et al 2008 supplement].
  
 
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Revision as of 01:27, 13 October 2018


3G pLac/ara-1

3G-compatible pLac/ara-1 dual input promoter. Flanked by prefix sticky end A and suffix sticky end B. Repressed by LacI and activated by arabinose/AraC. Intended for use in oscillator creation. Sequence originally from Lutz and Bujard 1997; later used for the creation of oscillator from Stricker et al 2008. Note that the upstream LacO site is required for functional repression via LacI (tetrameter) [See Stricker et al 2008 supplement].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 476
    Illegal XhoI site found at 443
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 556