Difference between revisions of "Part:BBa K2868028"

Revision as of 00:54, 13 October 2018

4xCBD Fusion Protein Expression System

T7 promoter, a salI cut-site, then a start codon then a Flag, Lumio, 6x polyhisitine tag, followed by Bacillus circulans chitin binding domain, flexible linker, S. griseus chitin binding domain, then another linker, followed by Bacillus circulans chitin binding domain, flexible linker, S. griseus chitin binding domain, stop codon, a bglII cut-site, and finally t7 terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 809
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2868028 short</partinfo>
-T7 promorot, a salI cut-site, then a start codon then a Flag, Lumio, 6x polyhisitine tag, followed by Bacillus circulans chitin binding domain, flexible linker, S. griseus chitin binding domain, then another linker, followed by Bacillus circulans chitin binding domain, flexible linker, S. griseus chitin binding domain, stop codon, a bglII cut-site, and finally t7 terminator.
+T7 promoter, a salI cut-site, then a start codon then a Flag, Lumio, 6x polyhisitine tag, followed by Bacillus circulans chitin binding domain, flexible linker, S. griseus chitin binding domain, then another linker, followed by Bacillus circulans chitin binding domain, flexible linker, S. griseus chitin binding domain, stop codon, a bglII cut-site, and finally t7 terminator.
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