Difference between revisions of "Part:BBa K2688025"
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This corrected version is devoid of extra-sequences and is therefore convenient for a direct biobrick cloning of RBS-CDS under the control of J23108 promoter.
 
This corrected version is devoid of extra-sequences and is therefore convenient for a direct biobrick cloning of RBS-CDS under the control of J23108 promoter.
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'''For details on the process, see [[http://2018.igem.org/Team:GO_Paris-Saclay/Improve| http://2018.igem.org/Team:GO_Paris-Saclay/Improve]]'''
  
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2688025 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2688025 SequenceAndFeatures</partinfo>

Revision as of 19:00, 12 October 2018

J23108_corrected

J23108 promoter is part of the Chris Anderson’s collection of plasmid. The pSB1C3-BBa_J23108 was obtained by PCR mutagenesis of the pSB1C3-BBa_J23119.

This biobrick is a corrected version of the initial biobrick (BBa_J23108) obtained from (2018 – Plate 4 – 4C) position which contains extra-sequences (notably SpeI forbidden sites).

This corrected version is devoid of extra-sequences and is therefore convenient for a direct biobrick cloning of RBS-CDS under the control of J23108 promoter.

For details on the process, see http://2018.igem.org/Team:GO_Paris-Saclay/Improve


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]