Difference between revisions of "Part:BBa K2656200"

Revision as of 17:05, 12 October 2018

pBIOBRICKATOR

pSB1C3 backbone modified to receive transcriptional units created in alpha 1 plasmids of Golden Braid 3.0 in a Golden Gate reaction using BsmbI endonuclease. It contains a red transcriptional unit in the cloning site for the screening.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found at 5
Plasmid lacks a suffix.
Illegal SpeI site found at 921
Illegal PstI site found at 935
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5
Illegal NheI site found at 44
Illegal NheI site found at 67
Illegal SpeI site found at 921
Illegal PstI site found at 935
Illegal NotI site found at 11
Illegal NotI site found at 928
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5
Illegal XhoI site found at 1952
Illegal XhoI site found at 2844
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 5
Illegal suffix found at 921
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 5
Plasmid lacks a suffix.
Illegal XbaI site found at 20
Illegal SpeI site found at 921
Illegal PstI site found at 935
Illegal AgeI site found at 653
Illegal AgeI site found at 765
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2656200 short</partinfo>
-Backbone pSB1C3 modified to receive transcriptional units created in alpha 1 plasmids of Golden Braid 3.0 in a Golden Gate reaction using BsmbI endonuclease. It contains a red transcriptional unit in the cloning site for the screening.
+pSB1C3 backbone modified to receive transcriptional units created in alpha 1 plasmids of Golden Braid 3.0 in a Golden Gate reaction using BsmbI endonuclease. It contains a red transcriptional unit in the cloning site for the screening.
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