Difference between revisions of "Part:BBa K2686005:Experience"
Line 1: Line 1:
−
 
+
The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing. The part was then amplified out of the pET vector and inserted into the pSB1C3 plasmid backbone, and submitted. There was not enough time to perform Sanger sequencing, but colony PCR gave us the correct insert size.
 
__NOTOC__
 
__NOTOC__
  
Line 15: Line 15:
 
<I>m_houmani_EPFL2018</I>
 
<I>m_houmani_EPFL2018</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
−
The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing. The part was then amplified out of the pET vector and inserted into the pSB1C3 plasmid backbone, and submitted. There was not enough time to perform Sanger sequencing, but colony PCR gave us the correct insert size.
+
 
 
|};
 
|};
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K2686005 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K2686005 EndReviews</partinfo>

Revision as of 16:49, 12 October 2018

The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing. The part was then amplified out of the pET vector and inserted into the pSB1C3 plasmid backbone, and submitted. There was not enough time to perform Sanger sequencing, but colony PCR gave us the correct insert size.


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing.

Applications of BBa_K2686005

User Reviews

UNIQee020bcbf68c9e74-partinfo-00000000-QINU UNIQee020bcbf68c9e74-partinfo-00000001-QINU