Difference between revisions of "Part:BBa K2656012"
Line 6: Line 6:
 
Using the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol], it can be combined with other GB adapted basic parts, such as the ones from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection], to assemble transcriptional units in a single one-pot reaction.  
 
Using the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol], it can be combined with other GB adapted basic parts, such as the ones from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection], to assemble transcriptional units in a single one-pot reaction.  
  
−
Characterization of the this part was performed with the transcriptional unit [https://parts.igem.org/Part:BBa_K2656104 BBa_K2656104], which was used in a comparative RBS expression experiment with composite parts [https://parts.igem.org/Part:BBa_K2656100 BBa_K265610 ] and [https://parts.igem.org/Part:BBa_K2656101 BBa_K2656101].  
+
Characterization of the this part was performed with the transcriptional unit [https://parts.igem.org/Part:BBa_K2656104 BBa_K2656104], which was used in a comparative RBS expression experiment with composite parts [https://parts.igem.org/Part:BBa_K2656100 BBa_K265610] and [https://parts.igem.org/Part:BBa_K2656101 BBa_K2656101].  
 
They all were assembled in a Golden Braid alpha1 plasmid using the same promoter, CDS and terminator:
 
They all were assembled in a Golden Braid alpha1 plasmid using the same promoter, CDS and terminator:
 
<html>
 
<html>
Line 22: Line 22:
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
<partinfo>BBa_K2656008 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K2656012 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K2656008 parameters</partinfo>
+
<partinfo>BBa_K2656012 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 16:28, 12 October 2018

Very Low Ribosome Binding Site J61101

BBa_K2656012 is the BBa_B0030 ribosome binding site standardized into the Golden Braid assembly method. Thus, it is strong RBS compatible both with the BioBrick and Golden Gate grammar.

Using the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol], it can be combined with other GB adapted basic parts, such as the ones from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection], to assemble transcriptional units in a single one-pot reaction.

Characterization of the this part was performed with the transcriptional unit BBa_K2656104, which was used in a comparative RBS expression experiment with composite parts BBa_K265610 and BBa_K2656101. They all were assembled in a Golden Braid alpha1 plasmid using the same promoter, CDS and terminator:

  • Promoter BBa_K2656004: the J23106 promoter in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
  • CDS BBa_K2656013: the BBa_K2656009 sfGFP sequence in its Golden Braid standardized version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
  • Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]