Difference between revisions of "Part:BBa K2718022"
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<partinfo>BBa_K2718022 short</partinfo> | <partinfo>BBa_K2718022 short</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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This clearly shows that we are able to produce significant amounts of chitinase. | This clearly shows that we are able to produce significant amounts of chitinase. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2718022 parameters</partinfo> | <partinfo>BBa_K2718022 parameters</partinfo> | ||
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Revision as of 14:16, 12 October 2018
IPTG inducible promoter with RBS Endochitinase 6-His
Usage and Biology
To verify the functionallity of this part we measured chitinase production after induction of E. coli (DH5a) at OD 0.8 with 1mm IPTG for 3 hours at 37°C.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 230
- 1000COMPATIBLE WITH RFC[1000]