Difference between revisions of "Part:BBa K2718022"

 
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===Usage and Biology===
 
===Usage and Biology===
  
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To verify the functionallity of this part we measured chitinase production after induction of E. coli (DH5a) at OD 0.8 with 1mm IPTG for 3 hours at 37°C.
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<html>
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<img src="https://static.igem.org/mediawiki/2018/f/fc/T--Aix-Marseille--Chitinase.jpg"/>
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<figcaption>Fig 1. (left) SDS-PAGE of chitinase production and (right) western blot revealed with anti-his antibody in NI (nor induced) and I (induced) E.coli cells.
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</figcaption></figure></html>
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This clearly shows that we are able to produce significant amounts of chitinase.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 14:15, 12 October 2018


IPTG inducible promoter with RBS Endochitinase 6-His

a

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]