Difference between revisions of "Part:BBa K2549031"
Line 3: Line 3:
  
 
This part is one of the response elements of our amplifier, also executing the combiner function. 8*ZF43.8 binding sites is assembled using two 4*ZF43.8 binding sites ([[Part:BBa_K2446006]]) with a biobrick scar between them. CMV ([[Part:BBa_K2549050]]) is a promotor which has a high-level constitutive expression. This part can switch off the expression of gene downstream after induced by our zinc finger-based transcription repressor.
 
This part is one of the response elements of our amplifier, also executing the combiner function. 8*ZF43.8 binding sites is assembled using two 4*ZF43.8 binding sites ([[Part:BBa_K2446006]]) with a biobrick scar between them. CMV ([[Part:BBa_K2549050]]) is a promotor which has a high-level constitutive expression. This part can switch off the expression of gene downstream after induced by our zinc finger-based transcription repressor.
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K2549031 SequenceAndFeatures</partinfo>
 +
  
 
<!-- Add more about the biology of this part here -->
 
<!-- Add more about the biology of this part here -->
 
===Biology===
 
===Biology===
−
====synthetic promotor operators regulated by artificial zinc finger-based transcription factors====
+
=====It works as we designed =====
−
JJ Collins et al have reported several synthetic promotor operators which can interact with artificial zinc finger-based transcription factors with high specificity and high orthogonality<ref>A synthetic biology framework for programming eukaryotic transcription functions. Khalil AS, Lu TK, Bashor CJ, ..., Joung JK, Collins JJ. Cell, 2012 Aug;150(3):647-58  PMID: 22863014; DOI: 10.1016/j.cell.2012.05.045</ref>.
+
interaction between ZF43.8-KRAB and 8*ZF43.8-CMV promotor
  
−
[[File:ZF2.jpg|none|540px|thumb|JJ Collins et al stated:''sTFs constructed from OPEN-engineered ZFs are orthogonal to one another. sTF43-8 activated noncognate Promoter21-16 due to the fortuitous creation of a sequence that is significantly similar to the binding sequence of 43-8, when the downstream BamHI restriction site is considered.'']]
+
@@@@
−
====interaction between ZF43.8-KRAB and 8*ZF43.8-CMV promotor====
+
  
−
<!-- -->
+
=====Synthetic promotor operators regulated by artificial zinc finger-based transcription factors=====
−
<span class='h3bb'>Sequence and Features</span>
+
Khalil AS et al have reported several synthetic promotor operators which can interact with artificial zinc finger-based transcription factors with high specificity and high orthogonality<ref>A synthetic biology framework for programming eukaryotic transcription functions. Khalil AS, Lu TK, Bashor CJ, ..., Joung JK, Collins JJ. Cell, 2012 Aug;150(3):647-58  PMID: 22863014; DOI: 10.1016/j.cell.2012.05.045</ref>.
−
<partinfo>BBa_K2549031 SequenceAndFeatures</partinfo>
+
 
 +
[[File:ZF2.jpg|none|540px|thumb|Khalil AS et al stated:''sTFs constructed from OPEN-engineered ZFs are orthogonal to one another. sTF43-8 activated noncognate Promoter21-16 due to the fortuitous creation of a sequence that is significantly similar to the binding sequence of 43-8, when the downstream BamHI restriction site is considered.'']]
  
  
Line 20: Line 25:
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2549031 parameters</partinfo>
 
<partinfo>BBa_K2549031 parameters</partinfo>
−
<!-- -->
+
  -->
−
 
+
 
===References===
 
===References===

Revision as of 14:13, 12 October 2018

8*ZF43.8-CMV

This part is one of the response elements of our amplifier, also executing the combiner function. 8*ZF43.8 binding sites is assembled using two 4*ZF43.8 binding sites (Part:BBa_K2446006) with a biobrick scar between them. CMV (Part:BBa_K2549050) is a promotor which has a high-level constitutive expression. This part can switch off the expression of gene downstream after induced by our zinc finger-based transcription repressor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Biology

It works as we designed

interaction between ZF43.8-KRAB and 8*ZF43.8-CMV promotor

@@@@

Synthetic promotor operators regulated by artificial zinc finger-based transcription factors

Khalil AS et al have reported several synthetic promotor operators which can interact with artificial zinc finger-based transcription factors with high specificity and high orthogonality[1].

Khalil AS et al stated:sTFs constructed from OPEN-engineered ZFs are orthogonal to one another. sTF43-8 activated noncognate Promoter21-16 due to the fortuitous creation of a sequence that is significantly similar to the binding sequence of 43-8, when the downstream BamHI restriction site is considered.


References

  1. ↑ A synthetic biology framework for programming eukaryotic transcription functions. Khalil AS, Lu TK, Bashor CJ, ..., Joung JK, Collins JJ. Cell, 2012 Aug;150(3):647-58 PMID: 22863014; DOI: 10.1016/j.cell.2012.05.045