Difference between revisions of "Part:BBa K2715011"

 
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<partinfo>BBa_K2715011 short</partinfo>
 
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==Usage and Biology==
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This basic part is the promoter portion of the ferredoxin regulatory region from the Gram-positive organism <i>Clostridium sporogenes</i>. Ferredoxins (from Latin ferrum: iron + redox, often abbreviated "fdx") are iron–sulfur proteins that mediate electron transfer in a range of metabolic reactions, and the ferredoxin gene is known is to highly expressed in <i>C. sporogenes</i>. This promoter has been demonstrated previously to be a strong promoter in clostridial species (ref).
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==Characterisation==
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This basic part was characterised as part of a composite part, and used as a promoter which functions in both the Gram-negative <i>E. coli</i> and the Gram-positive <i>Clostridium difficile</i>. The full native regulatory region driving thiolase expression in <i>C. sporogenes</i> is composed of this ferredoxin promoter [https://parts.igem.org/Part:BBa_K2715011 BBa_K2715011] and it's RBS [https://parts.igem.org/Part:BBa_K2715020 BBa_K2715020]. It's strength was assessed in <i>E. coli</i> using GFP as a reporter gene, the link to the characterisation data is provided below. A composite part was also assembled using <i>gusA</i> as a reporter gene, and this was used to assay its strength in <i>Clostridium difficile</i>. The composite part driving <i>gusA</i> is also listed below:
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GFP assay:
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[https://parts.igem.org/Part:BBa_K2715002 BBa_K2715002]
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GUS assay:
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[https://parts.igem.org/Part:BBa_K2715026 BBa_K2715026]
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===Usage and Biology===
 
  
 
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=References==

Revision as of 14:01, 12 October 2018


Constitutive promoter from C.sporogenes ferrodoxin gene

Usage and Biology

This basic part is the promoter portion of the ferredoxin regulatory region from the Gram-positive organism Clostridium sporogenes. Ferredoxins (from Latin ferrum: iron + redox, often abbreviated "fdx") are iron–sulfur proteins that mediate electron transfer in a range of metabolic reactions, and the ferredoxin gene is known is to highly expressed in C. sporogenes. This promoter has been demonstrated previously to be a strong promoter in clostridial species (ref).

Characterisation

This basic part was characterised as part of a composite part, and used as a promoter which functions in both the Gram-negative E. coli and the Gram-positive Clostridium difficile. The full native regulatory region driving thiolase expression in C. sporogenes is composed of this ferredoxin promoter BBa_K2715011 and it's RBS BBa_K2715020. It's strength was assessed in E. coli using GFP as a reporter gene, the link to the characterisation data is provided below. A composite part was also assembled using gusA as a reporter gene, and this was used to assay its strength in Clostridium difficile. The composite part driving gusA is also listed below:

GFP assay:

BBa_K2715002


GUS assay:

BBa_K2715026



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References=