Difference between revisions of "Part:BBa K2762010:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | We first codon optimized the rbcXS sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning. The sequence is then cloned into the pSB1C3 plasmid with EcoRI and SpeI. | |
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | Codon oprimized <i>Synechococcus elongatus</i> PCC7002. | |
| − | + | ||
===References=== | ===References=== | ||
Latest revision as of 13:55, 12 October 2018
PlacI-B0034-rbcX-B0034-rbcS-B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We first codon optimized the rbcXS sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning. The sequence is then cloned into the pSB1C3 plasmid with EcoRI and SpeI.
Source
Codon oprimized Synechococcus elongatus PCC7002.