Difference between revisions of "Part:BBa K2671000"

Revision as of 13:25, 12 October 2018

AraC-pBAD-mNG

Liu iGEM2017s plasmid with an improvement. A stop codon was mutated behind mNeonGreen (mNG). The original part was mNG fused to amyloid-β, where the fusion protein had its stop codon after amyloid-β. Another mutation was made in the GS-linker sequence where a SpeI-site was added and the sequence for amyloid-β was cleaved off. With these improvements mNG can now be used as a reporter in a fully functional operon with the pBAD-AraC inducible system. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1267
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

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 <partinfo>BBa_K2671000 short</partinfo>
-Liu iGEM2017s plasmid with an improvement. A stop codon was mutated behind mNeonGreen (mNG). The original part was mNG fused to amyloid-&#946;, where the fusion protein had its stop codon after amyloid-&#946;. Another mutation was made in the GS-linker sequence where a SpeI-site was added and the sequence for amyloid-β was cleaved off. With these improvements mNG can now be used as a reporter in a fully functional operone with the pBAD-AraC inducible system.
+Liu iGEM2017s plasmid with an improvement. A stop codon was mutated behind mNeonGreen (mNG). The original part was mNG fused to amyloid-&#946;, where the fusion protein had its stop codon after amyloid-&#946;. Another mutation was made in the GS-linker sequence where a SpeI-site was added and the sequence for amyloid-β was cleaved off. With these improvements mNG can now be used as a reporter in a fully functional operon with the pBAD-AraC inducible system.
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 ===Usage and Biology===