Difference between revisions of "Part:BBa K2762001:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
-
+
We first codon optimized the rbcX sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning. The sequence is then cloned into the pSB1C3 plasmid with HindIII and SpeI.
  
  

Revision as of 13:06, 12 October 2018


Assembly chaperone of ribulose-bisphosphate carboxylase/oxygenase rbcX


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We first codon optimized the rbcX sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning. The sequence is then cloned into the pSB1C3 plasmid with HindIII and SpeI.


Source

-

References