Difference between revisions of "Part:BBa K2558212"

 
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<partinfo>BBa_K2558212 short</partinfo>
 
<partinfo>BBa_K2558212 short</partinfo>
  
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This construct is composed of constantly expressed luxR,  and lux pR controlled sfGFP as our reporter. We aim to use this construct to help us test lux pR system and AHL induction efficiency. We designed this device along with K2558211, which is basically the same, except for the different proporties of the the lux pR and the lux pR-HS promotor.  
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This construct is composed of constantly expressed luxR,  and lux pR-HS (https://parts.igem.org/Part:BBa_K2558001) controlled sfGFP as our reporter. We aim to use this construct to help us test lux pR system and AHL induction efficiency. We designed this device along with K2558211, which is the same except for the different properties of the the lux pR and the lux pR-HS promotor.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:23, 12 October 2018


lux pR-HS test device

This construct is composed of constantly expressed luxR, and lux pR-HS (https://parts.igem.org/Part:BBa_K2558001) controlled sfGFP as our reporter. We aim to use this construct to help us test lux pR system and AHL induction efficiency. We designed this device along with K2558211, which is the same except for the different properties of the the lux pR and the lux pR-HS promotor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1074
    Illegal NheI site found at 1097
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 177