Difference between revisions of "Part:BBa K2547004"

Revision as of 11:21, 12 October 2018

Carbonic anhydrase 2 (L203K)

This part is the coding sequence (CDS) of human carbonic anhydrase (CA2), because the carbonic anhydrase (CA2) extracted in human body has the fastest reaction rate at 37 ° C, and loses at 50 ° C. Live, so the DNA molecule was mutated and found that when the amino acid encoded by the 203th codon was mutated from leucine to lysine, the carbonic anhydrase produced had higher thermal stability and was encoded. The addition of a histidine tag (His-Tag) following the sequence (CDS) facilitates the purification of the carbonic anhydrase protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2547004 short</partinfo>
-This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because the carbonic anhydrase (CA-II) extracted in human body has the fastest reaction rate at 37 ° C, and loses at 50 ° C. Live, so the DNA molecule was mutated and found that when the amino acid encoded by the 203th codon was mutated from leucine to lysine, the carbonic anhydrase produced had higher thermal stability and was encoded. The addition of a histidine tag (His-Tag) following the sequence (CDS) facilitates the purification of the carbonic anhydrase protein.
+This part is the coding sequence (CDS) of human carbonic anhydrase (CA2), because the carbonic anhydrase (CA2) extracted in human body has the fastest reaction rate at 37 ° C, and loses at 50 ° C. Live, so the DNA molecule was mutated and found that when the amino acid encoded by the 203th codon was mutated from leucine to lysine, the carbonic anhydrase produced had higher thermal stability and was encoded. The addition of a histidine tag (His-Tag) following the sequence (CDS) facilitates the purification of the carbonic anhydrase protein.
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