Difference between revisions of "Part:BBa K2675015"

Revision as of 09:41, 12 October 2018

Synthetic RBS designed for sfGFP

This part is a synthetic RBS designed for sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) using the Salis Lab RBS calculator v2.0 [1, 2].

Usage and Biology

This RBS was used to drive the expression of sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) under the control of the pAimX short promoter (BBa_K2675021) or its derivative (BBa_K2675022) in the composite parts BBa_K2675051, BBa_K2675052, BBa_K2675058, BBa_K2675059, BBa_K2675061 & BBa_K2675062.

Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
Translation Initiation Rate (au)	:	202865,54
dG_total (kcal/mol)	:	-11,34
dG_mRNA_rRNA (kcal/mol)	:	-20,08
dG_spacing (kcal/mol)	:	0
dG_standby (kcal/mol)	:	0
dG_start (kcal/mol)	:	-2,76
dG_mRNA (kcal/mol)	:	-12,28
Accuracy (warnings)	:	OK

Sequence and Features