Difference between revisions of "Part:BBa K2856002"

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===Usage and Biology===
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===Usage and Biology===  
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S-adenosylmethionine synthetase(MetK) is an enzyme involved and responded to synthetic reaction of GSH.In this
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reaction,one Cys and one Glu are converted to one γ-GC,then one γ-GC and one Gly are converted to one GSH.(Figure 1) The Lactococcus Lactis NZ9000 has inability to synthesis GSH.In our project,we construct a plasmid harboring gshF in order to produnce GSH in Lactococcus Lactis NZ9000.
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[[File:T--H14Z1 Hangzhou--Reaction_metK.jpeg|500px|thumb|centre| <p>'''Figure. 1  Enzymatic reaction catalyzed by metK'''</p>]]
 
[[File:T--H14Z1 Hangzhou--Reaction_metK.jpeg|500px|thumb|centre| <p>'''Figure. 1  Enzymatic reaction catalyzed by metK'''</p>]]

Revision as of 08:44, 12 October 2018


Modification of S-adenosylmethionine synthetase (MetK)

The BBa_K2856001 harbors a coding sequence of S-adenosylmethionine synthetase(MetK) derived from Lactococcus Lactis NZ9000 genome.The MetK protein catalyzes methionine and ATP to S-adenosyl methionine.


Usage and Biology

S-adenosylmethionine synthetase(MetK) is an enzyme involved and responded to synthetic reaction of GSH.In this reaction,one Cys and one Glu are converted to one γ-GC,then one γ-GC and one Gly are converted to one GSH.(Figure 1) The Lactococcus Lactis NZ9000 has inability to synthesis GSH.In our project,we construct a plasmid harboring gshF in order to produnce GSH in Lactococcus Lactis NZ9000.


Figure. 1 Enzymatic reaction catalyzed by metK

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]