Difference between revisions of "Part:BBa K2789009:Design"
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+ | We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102) |
Revision as of 05:44, 12 October 2018
optimized Ccas from BBa_K68102
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1310
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 715
Illegal AgeI site found at 1720
Illegal AgeI site found at 1915 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We have optimized the code in the Ccas and we deleted the EcoRI restriction site in the Ccas for future DNA ligation and digestion.
Source
initial Ccas from BBa_K68102
References
We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102)