Difference between revisions of "Part:BBa K2789003:Design"

Revision as of 05:43, 12 October 2018

lac operator+rbs+Ccas+terminator+Ccar+lac operator+rbs+terminator+cpcg+non gate+rbs+gfp+termiantor

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1393
Illegal BamHI site found at 2973
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 798
Illegal AgeI site found at 1803
Illegal AgeI site found at 1998
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 5242

Design Notes

We have optimized the code in the Ccas and Ccar. And we deleted the EcoRI restriction site in the Ccas for future DNA ligation and digestion.

Source

All the parts come from igem registry resources. Ccas: BBa_K608102 Ccar: BBa_K592002 Lac operator: BBa_C0010, BBa_C0012 double terminator: BBa_B0015 non gate: BBa_Q04400 rbs:BBa_B0034 gfp: BBa_K082003

References

We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102)

Revision as of 01:50, 6 October 2018 (view source) Registry (Talk \| contribs)		Revision as of 05:43, 12 October 2018 (view source) Duliaoqi (Talk \| contribs) (→‎References) Newer edit →
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	===References===		===References===
		+	We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102)