Difference between revisions of "Part:BBa K2683003:Design"
Kristiturton (Talk | contribs) (→References) |
Kristiturton (Talk | contribs) (→References) |
||
| Line 16: | Line 16: | ||
===References=== | ===References=== | ||
| − | [1] | + | [1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664 |
| + | |||
[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138 | [2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138 | ||
Revision as of 03:21, 12 October 2018
MS2 Protein with Polyarginine Peptide
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 63
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A polyarginine cell penetrating peptide was chosen due to the non-specific nature of the peptide. Available research on cell penetrating peptides for bacterial systems tend to be specific for the peptidoglycan of the bacterial cell wall. Therefore, using these types of peptides would need to be expressed in a different model system as it would likely kill the E.coli we wished to express the capsid in.
Source
The MS2 capsid is originally from the MS2 bacteriphage. Our sequence was synthesized as a g-block
References
[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664
[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138