Difference between revisions of "Part:BBa K2570021"
Line 5: Line 5:
 
RpoS is a stationary-phase and stress response sigma factor in ''E. coli'' and many other bacteria. One environmental cue that increases RpoS synthesis is low temperature (below 37°C). This increase is completely dependent upon ''dsrA'', a small noncoding RNA[2]. ''dsrA'' was shown to stimulate RpoS translation by pairing with a portion of the mRNA upstream of the RpoS translation start that can pair with and occlude the ribosome-binding site of the transcript [3]. ''dsrA'' is more abundant in ''E. coli'' at low growth temperatures than at higher temperatures, resulting in the increased RpoS expression at low temperatures [4]. Temperature affects both the synthesis and the stability of ''dsrA'', leading to thermocontrol of RpoS translation.
 
RpoS is a stationary-phase and stress response sigma factor in ''E. coli'' and many other bacteria. One environmental cue that increases RpoS synthesis is low temperature (below 37°C). This increase is completely dependent upon ''dsrA'', a small noncoding RNA[2]. ''dsrA'' was shown to stimulate RpoS translation by pairing with a portion of the mRNA upstream of the RpoS translation start that can pair with and occlude the ribosome-binding site of the transcript [3]. ''dsrA'' is more abundant in ''E. coli'' at low growth temperatures than at higher temperatures, resulting in the increased RpoS expression at low temperatures [4]. Temperature affects both the synthesis and the stability of ''dsrA'', leading to thermocontrol of RpoS translation.
  
−
Then,we use the GFP[https://parts.igem.org/wiki/index.php?title=Part:BBa_B0040 BBa_B0040] to characterize the intensity of the temperature-controlled promoter.
+
Then,we use the GFP[https://parts.igem.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040] to characterize the intensity of the temperature-controlled promoter.
  
  

Revision as of 03:00, 12 October 2018

dsrA+B0034+GFP+B0032+mazF

Temperature is one environmental variable that all organisms must deal with. Besides the direct effect of temperature on enzymatic reactions, temperature response involves remodeling of the expression pattern of genes, affecting transcription, RNA stability, translation efficiency, and/or proteolysis. RpoS is a stationary-phase and stress response sigma factor in E. coli and many other bacteria. One environmental cue that increases RpoS synthesis is low temperature (below 37°C). This increase is completely dependent upon dsrA, a small noncoding RNA[2]. dsrA was shown to stimulate RpoS translation by pairing with a portion of the mRNA upstream of the RpoS translation start that can pair with and occlude the ribosome-binding site of the transcript [3]. dsrA is more abundant in E. coli at low growth temperatures than at higher temperatures, resulting in the increased RpoS expression at low temperatures [4]. Temperature affects both the synthesis and the stability of dsrA, leading to thermocontrol of RpoS translation.

Then,we use the GFPBBa_E0040 to characterize the intensity of the temperature-controlled promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 721