Difference between revisions of "Part:BBa K2608006:Design"

 
(Design Notes)
 
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===Design Notes===
 
===Design Notes===
For expression efficiently,it was truncated.
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For expression efficiently,it was truncated as 173aa and codons were optimized.
 
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===Source===
 
===Source===

Latest revision as of 01:27, 12 October 2018


truncated and optimized HCV core protein (173 aa) with part of cspA 5UTR sequence and TEE, 6xHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 273
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 490
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For expression efficiently,it was truncated as 173aa and codons were optimized.

Source

HCV core protein cDNA.

References