Difference between revisions of "Part:BBa K2683025"
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<partinfo>BBa_K2683025 short</partinfo> | <partinfo>BBa_K2683025 short</partinfo> | ||
− | IF3 | + | Initiation Factor 3 (IF3) is a protein that aids the initiation of protein synthesis (peptide chain initiation). IF3 binds to the 30S subunit of the ribosome, causing ribosomal subunit interactions to change in favour for starting protein synthesis [1]. This part is codon optimized for <i> Escherichia coli </i>, has a N-terminal his tag for purification and has a T7promoter, Medium strength RBS and double terminator. These initial designs were by the 2017 Lethbridge iGEM team (http://2017.igem.org/Team:Lethbridge). |
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+ | This part was improved by placing the part into the pSB1C3 plasmid. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 01:03, 12 October 2018
Initiation Factor 3 (IF3) in pSb1C3
Initiation Factor 3 (IF3) is a protein that aids the initiation of protein synthesis (peptide chain initiation). IF3 binds to the 30S subunit of the ribosome, causing ribosomal subunit interactions to change in favour for starting protein synthesis [1]. This part is codon optimized for Escherichia coli , has a N-terminal his tag for purification and has a T7promoter, Medium strength RBS and double terminator. These initial designs were by the 2017 Lethbridge iGEM team (http://2017.igem.org/Team:Lethbridge).
This part was improved by placing the part into the pSB1C3 plasmid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]