Difference between revisions of "Part:BBa K2694001:Design"
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===Design Notes===
 
===Design Notes===
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This part was designed according to a paper by Wilhelm et al. in the Journal of Bacteriology in 1999 and the corresponding <a href=“https://www.ebi.ac.uk/ena/data/view/AF005091”>EBI sequence</a>. This paper included the DNA sequence that coded the esterase, which we codon optimized and removed the illegal cut sites via silent mutations (i.e., the amino acid sequence was unchanged).  
+
This part was designed according to a paper by Wilhelm et al. in the Journal of Bacteriology in 1999 and the corresponding [https://www.ebi.ac.uk/ena/data/view/AF005091 EBI sequence]. This paper included the DNA sequence that coded the esterase, which we codon optimized and removed the illegal cut sites via silent mutations (i.e., the amino acid sequence was unchanged).  
  
 
===Source===
 
===Source===

Revision as of 00:44, 12 October 2018


EstA from Pseudomonas aeruginosa


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 215
    Illegal BglII site found at 362
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 145
    Illegal NgoMIV site found at 601
    Illegal NgoMIV site found at 739
    Illegal NgoMIV site found at 1279
    Illegal NgoMIV site found at 1648
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed according to a paper by Wilhelm et al. in the Journal of Bacteriology in 1999 and the corresponding EBI sequence. This paper included the DNA sequence that coded the esterase, which we codon optimized and removed the illegal cut sites via silent mutations (i.e., the amino acid sequence was unchanged).

Source

Wilhelm et al., J Bacteriol, 1999, 6977-86. Synthesized through BioBasic.

References