Difference between revisions of "Part:BBa K2683023"

Latest revision as of 00:42, 12 October 2018

Valine tRNA Synthetase (ValRS) in pSB1C3

Valine tRNA synthetase is responsible for attaching the valine amino acid onto its tRNA to form an aminoacyl-tRNA [1]. The part has been codon optimized for use in Escherichia coli , contains a C-terminal hexahistidine tag with a serine glycine linker and has a T7 Promoter, medium strength RBS and double terminator. These original designs were done by the 2017 Lethbridge iGEM team (http://2017.igem.org/Team:Lethbridge).

This part has been improved by inserting it into the pSB1C3 plasmid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2661

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2683023 short</partinfo>
-ValRs now in the pSb1C3 standard
+Valine tRNA synthetase is responsible for attaching the valine amino acid onto its tRNA to form an aminoacyl-tRNA [1]. The part has been codon optimized for use in <i> Escherichia coli </i>,  contains a C-terminal hexahistidine tag with a serine glycine linker and has a T7 Promoter, medium strength RBS and double terminator. These original designs were done by the 2017 Lethbridge iGEM team (http://2017.igem.org/Team:Lethbridge).
+This part has been improved by inserting it into the pSB1C3 plasmid.
 <!-- Add more about the biology of this part here