Difference between revisions of "Part:BBa K2683022"

Revision as of 00:20, 12 October 2018

Tryptophan tRNA Synthetase (TrpRS) in pSb1C3

Tryptophan tRNA synthetase is the protein responsible for attaching Tryptophan onto its tRNA to form an aminoacyl-tRNA. This part is codon optimized for use in Escherichia coli , contains a C-terminal hexahistidine tag with a serine glycine linker and has a T7 Promoter, medium strength RBS and double terminator. These designs were originally done by the Lethbridge iGEM team 2017 (http://2017.igem.org/Team:Lethbridge). The part was initially characterized in the pUC57 plasmid (https://parts.igem.org/Part:BBa_K2443019).

This part was improved by the placing the part into the pSB1C3 plasmid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 705
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2683022 short</partinfo>
-TrpRs now in the pSb1C3 biobrick standard
+Tryptophan tRNA synthetase is the protein responsible for attaching Tryptophan onto its tRNA to form an aminoacyl-tRNA. This part is codon optimized for use in <i> Escherichia coli </i>,  contains a C-terminal hexahistidine tag with a serine glycine linker and has a T7 Promoter, medium strength RBS and double terminator. These designs were originally done by the Lethbridge iGEM team 2017 (http://2017.igem.org/Team:Lethbridge). The part was initially characterized in the pUC57 plasmid (https://parts.igem.org/Part:BBa_K2443019).
+This part was improved by the placing the part into the pSB1C3 plasmid.
 <!-- Add more about the biology of this part here