Difference between revisions of "Part:BBa K2683021"

Revision as of 00:08, 12 October 2018

Serine tRNA Synthetase (SerRS) in pSB1C3

Serine tRNA synthetase is a protein responsible for attaching serine onto its tRNA to form an aminoacyl-tRNA [1]. The part is codon optimized for use in Escherichia coli , contains a C-terminal hexahistidine tag with a serine glycine linker and has a T7 Promoter, medium strength RBS and double terminator. These designs were originally done by the Lethbridge iGEM team 2017 (http://2017.igem.org/Team:Lethbridge). The part was characterized in the pUC57 plasmid (https://parts.igem.org/Part:BBa_K2443017).

The part was improved by placing it within the pSB1C3 plasmid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 172

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2683021 short</partinfo>
-Serine tRNA synthetase is a protein responsible for attaching serine onto its tRNA to form an aminoacyl-tRNA. The part is codon optimized for use in <i> Escherichia coli </i>, contains a C-terminal hexahistidine tag with a serine glycine linker and has a  T7 Promoter, medium strength RBS and double terminator. These designs were originally done by the Lethbridge iGEM team 2017 (http://2017.igem.org/Team:Lethbridge). The part was characterized in the pUC57 plasmid
+Serine tRNA synthetase is a protein responsible for attaching serine onto its tRNA to form an aminoacyl-tRNA [1]. The part is codon optimized for use in <i> Escherichia coli </i>, contains a C-terminal hexahistidine tag with a serine glycine linker and has a  T7 Promoter, medium strength RBS and double terminator. These designs were originally done by the Lethbridge iGEM team 2017 (http://2017.igem.org/Team:Lethbridge). The part was characterized in the pUC57 plasmid
 (https://parts.igem.org/Part:BBa_K2443017).