Difference between revisions of "Part:BBa K2694000:Experience"

Revision as of 23:55, 11 October 2018

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Applications of BBa_K2694000

User Reviews

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[http://2018.igem.org/Team:NDC-HighRiverAB NDC-HighRiverAB]

A comparison of 4-nitrophenyl palmitate and 4-nitrophenyl octanoate

Figure 1: The top row shows centrifuge tubes containing the 4-nitrohenol ester reaction mixture for the palmitate (a) and octanoate (b) esters with no cells added. The middle row shows the response for a negative control where DH5⍺ cells without any plasmid were added to reaction mixtures containing 4-nitrophenyl-plamitate (c) or 4-nitrohenyl-octanoate (d). The bottom row shows the response for the BBa_K2694000 circuit transformed into DH5⍺ cells when added to reaction mixtures containing 4-nitrohenyl-plamitate (e) or 4-nitrohenyl-octanoate (f). All reaction mixtures were 1mM of the nitrophenyl ester with 1% v/v Triton X-100 in 0.1M pH 7 phosphate buffer.

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 <img src="https://static.igem.org/mediawiki/2018/7/7e/T--NDC-HighRiverAB--4nitro.jpeg" alt="A comparison of 4-nitrophenyl palmitate and 4-nitrophenyl octanoate">
-<p> Figure 1: The top row shows centrifuge tubes containing the 4-nitrohenol ester reaction mixture for the palmitate (a) and octanoate (b) esters with no cells added. The middle row shows the response for a negative control where DH5⍺ cells without any plasmid were added to reaction mixtures containing 4-nitrohenyl-plamitate (c) or 4-nitrohenyl-octanoate (d). The bottom row shows the response for the BBa_K2694000 circuit transformed into DH5⍺ cells when added to reaction mixtures containing 4-nitrohenyl-plamitate (e) or 4-nitrohenyl-octanoate (f). All reaction mixtures were 1mM of the nitrophenyl ester with 1% v/v Triton X-100 in 0.1M pH 7 phosphate buffer.</p>
+<p> Figure 1: The top row shows centrifuge tubes containing the 4-nitrohenol ester reaction mixture for the palmitate (a) and octanoate (b) esters with no cells added. The middle row shows the response for a negative control where DH5⍺ cells without any plasmid were added to reaction mixtures containing 4-nitrophenyl-plamitate (c) or 4-nitrohenyl-octanoate (d). The bottom row shows the response for the BBa_K2694000 circuit transformed into DH5⍺ cells when added to reaction mixtures containing 4-nitrohenyl-plamitate (e) or 4-nitrohenyl-octanoate (f). All reaction mixtures were 1mM of the nitrophenyl ester with 1% v/v Triton X-100 in 0.1M pH 7 phosphate buffer.</p>
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