Difference between revisions of "Part:BBa K2683020"

Revision as of 23:54, 11 October 2018

Phenylalanine tRNA Synthetase alpha subunit (PheRS alpha) in pSB1C3

Phenylalanine tRNA synthetase (PheRS) is responsible for attaching phenylalanine onto its tRNA to form an aminoacyl-tRNA. PheRS has both a alpha and beta subunit. The part is codon optimized for use in Escherichia coli , contains a N-terminal hexahistidine tag with a serine glycine linker and is under the regulation of a T7 Promoter, medium strength RBS and double terminator. The part was originally designed by the Lethbridge iGEM team 2017 (http://2017.igem.org/Team:Lethbridge) and was initially characterized in the pUC57 plasmid (https://parts.igem.org/Part:BBa_K2443014).

The part was improved by placing it into the pSB1C3 plasmid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2683020 short</partinfo>
-PheRS now in the pSb1C3 plasmid standard
+Phenylalanine tRNA synthetase (PheRS) is responsible for attaching phenylalanine onto its tRNA to form an aminoacyl-tRNA. PheRS has both a alpha and beta subunit. The part is codon optimized for use in <i>Escherichia coli </i>, contains a N-terminal hexahistidine tag with a serine glycine linker and is under the regulation of a T7 Promoter, medium strength RBS and double terminator. The part was originally designed by the Lethbridge iGEM team 2017 (http://2017.igem.org/Team:Lethbridge) and was initially characterized in the pUC57 plasmid (https://parts.igem.org/Part:BBa_K2443014).
+The part was improved by placing it into the pSB1C3 plasmid.
 <!-- Add more about the biology of this part here