Difference between revisions of "Part:BBa K2683018:Design"

(Source)
(References)
 
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===References===
 
===References===
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[1] Woese, C., Olsen, G., Ibba, M., and Soll, D. (2000) Aminoacyl-tRNA synthetases, the genetic code and the evolutionary process. Microbiology and Molecular Biology Reviews. 64, 202-236

Latest revision as of 23:31, 11 October 2018


Cysteine tRNA synthetase (CysRS) in pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1065
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 931


Design Notes

This subparts and coding sequence are codon optimized/designed for expression in E.coli .

Source

https://parts.igem.org/Part:BBa_K2443004

References

[1] Woese, C., Olsen, G., Ibba, M., and Soll, D. (2000) Aminoacyl-tRNA synthetases, the genetic code and the evolutionary process. Microbiology and Molecular Biology Reviews. 64, 202-236