Difference between revisions of "Part:BBa K2591020"

Revision as of 17:39, 11 October 2018

A gRNA designed to PORCN, an essential gene to secretion of the canonical wnt protein

Single-gRNA is a small RNA which help CRISRR-Cas protein family to target the exogenous sequence, and assist the prokaryotes to defend phages. Nowadays, the artificial gRNA can achieve the modification casually in the gene level and is a excellent way to introduce mutation. In our project, we design this gRNA for porcupine O-acyltransferase (PORCN), an essential enzyme to function at the Wnt3A protein secretion. Therefore, we use the gRNA to target PORCN, we can inhibit the donor cell to secret the Wnt3A protein normally.

Sequence and Features