Difference between revisions of "Part:BBa K2623023"

Revision as of 13:04, 11 October 2018

Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini

It’s another form of the BBa_K2623021 designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). The only difference is that we inserted SpyTag to C-termini of OmpA protein(instead of the N-termi in BBa_K2623021). Through the fluorescence intensity of GFP, we can evaluate the efficiency of their transport to the outer membrane and OMVs.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 65
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2623023 short</partinfo>
-It’s another form of the BBa_K2623001.This part was designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We inserted SpyTag to its N-termini and SpyCather to L7Ae (BBa_K2623026) in order to anchore L7Ae to outer membrane through the bioconjugation of the SpyTag/SpyCatcher system. Through the fluorescence of GFP, we can evaluate the efficiency of their transport to the outer membrane and OMVs.
+It’s another form of the BBa_K2623021 designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). The only difference is that we inserted SpyTag to C-termini of OmpA protein(instead of the N-termi in BBa_K2623021).  Through the fluorescence intensity of GFP, we can evaluate the efficiency of their transport to the outer membrane and OMVs.
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