Difference between revisions of "Part:BBa K2665015"

Revision as of 10:07, 11 October 2018

TDH3-AVP1-6xHis-CYC This gene codes Arabidopsis vacuolar proton-pumping pyrophos- phatase (H+-PPase) gene (AVP1). AVP1 uses energy derived from the hydrolysis of cytosolic inorganic pyrophosphate (PPi) to orthophosphate (Pi) to actively pump protons into vacuoles. This increases acidification in vacuole, which establishes a difference in electrochemical potential for H+ across the tonoplast. The difference is used by other vacuolar transporters, such as Na+/H+ antiporters. By this process, transgenic plants overexpressing AVP1 can facilitate sequestration of Na+ into vacuole from cytoplasm and survive under high salt conditions

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1341
Illegal BglII site found at 2598
Illegal BamHI site found at 2394
Illegal BamHI site found at 3030
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2690
Illegal BsaI.rc site found at 2880

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2665015 short</partinfo>
+This gene codes Arabidopsis vacuolar proton-pumping pyrophos- phatase (H+-PPase) gene (AVP1). AVP1 uses energy derived from the hydrolysis of cytosolic inorganic pyrophosphate (PPi) to orthophosphate (Pi) to actively pump protons into vacuoles. This increases acidification in vacuole, which establishes a difference in electrochemical potential for H+ across the tonoplast. The difference is used by other vacuolar transporters, such as Na+/H+ antiporters. By this process, transgenic plants overexpressing AVP1 can facilitate sequestration of Na+ into vacuole from cytoplasm  and survive under high salt conditions
-a
 <!-- Add more about the biology of this part here