Difference between revisions of "Part:BBa K2889000:Experience"
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===Applications of BBa_K2889000=== | ===Applications of BBa_K2889000=== | ||
| − | Cell migration is a central process in the development and maintenance of tumor. We cloned the full length of IL7-AS-S2 into PCDNA3.1 and transfected the plasmid to 786-O cells.Through in vitro scratch wound healing assay, overexpression of IL7-AS-S2 promoted cell migration of 786-O cells.Overexpression of IL7-AS-S2 increased the migration of 786-O cells (Fig. 1 and 2). These results suggested that IL7-AS-S2 contain key structural domains. | + | 1.cell migration |
| + | 1.1 Cell migration is a central process in the development and maintenance of tumor. We cloned the full length of IL7-AS-S2 into PCDNA3.1 and transfected the plasmid to 786-O cells.Through in vitro scratch wound healing assay, overexpression of IL7-AS-S2 promoted cell migration of 786-O cells.Overexpression of IL7-AS-S2 increased the migration of 786-O cells (Fig. 1 and 2). These results suggested that IL7-AS-S2 contain key structural domains. | ||
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| + | https://static.igem.org/mediawiki/parts/8/8c/IL7-AS-AS2.jpg | ||
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| + | https://static.igem.org/mediawiki/parts/a/ad/IL7-AS-S2-cell_migration.jpg | ||
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| + | 1.2 In order to investigate whether cell migration induced by IL7-AS-S2 depends on the amount of IL7-AS-S2 transfection. We transfected the different concentration of IL7-AS-S2 () into 786-0 cells.(Fig. 3 and 4) | ||
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| + | https://static.igem.org/mediawiki/parts/6/6a/Different_concentration_of_IL7-AS-S2.jpg | ||
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| + | https://static.igem.org/mediawiki/parts/b/bb/Different_concentration_of_IL7-AS-S2-.jpg | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 08:01, 11 October 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2889000
1.cell migration 1.1 Cell migration is a central process in the development and maintenance of tumor. We cloned the full length of IL7-AS-S2 into PCDNA3.1 and transfected the plasmid to 786-O cells.Through in vitro scratch wound healing assay, overexpression of IL7-AS-S2 promoted cell migration of 786-O cells.Overexpression of IL7-AS-S2 increased the migration of 786-O cells (Fig. 1 and 2). These results suggested that IL7-AS-S2 contain key structural domains.
1.2 In order to investigate whether cell migration induced by IL7-AS-S2 depends on the amount of IL7-AS-S2 transfection. We transfected the different concentration of IL7-AS-S2 () into 786-0 cells.(Fig. 3 and 4)
User Reviews
UNIQ7d611b88831a9a30-partinfo-00000000-QINU UNIQ7d611b88831a9a30-partinfo-00000001-QINU