Difference between revisions of "Part:BBa K2685006:Design"
| Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K2685006 short</partinfo> | <partinfo>BBa_K2685006 short</partinfo> | ||
| + | This parts can provide tetracycline tolerance to our constructive detecting chasis which including tetA and tetO genes. | ||
| + | TetA encodes the tetracycline efflux pump protein, which selectively transports tetracycline from the cytoplasm to the periplasm in exchange for protons, thus limiting tetracycline's access to ribosomes within cells.They are proton power dependent, single polypeptide, drug-specific efflux pump, and it belong to the main promoting factor superfamily. | ||
| + | We also design some single sites mutation comparing with the original sequence that we have got from the biobrick bank. | ||
<partinfo>BBa_K2685006 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2685006 SequenceAndFeatures</partinfo> | ||
Revision as of 06:30, 11 October 2018
TetO+TetA
This parts can provide tetracycline tolerance to our constructive detecting chasis which including tetA and tetO genes. TetA encodes the tetracycline efflux pump protein, which selectively transports tetracycline from the cytoplasm to the periplasm in exchange for protons, thus limiting tetracycline's access to ribosomes within cells.They are proton power dependent, single polypeptide, drug-specific efflux pump, and it belong to the main promoting factor superfamily. We also design some single sites mutation comparing with the original sequence that we have got from the biobrick bank.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1043
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 897
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 343
Illegal NgoMIV site found at 503
Illegal NgoMIV site found at 871 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
detecting tetracycline and pump out the tetracycline
Source
from the kit plate