Difference between revisions of "Part:BBa K2683017"

Revision as of 05:15, 11 October 2018

Release Factor 2 in pSB1C3

Release factor 2 i,proved with insertion into pSB1C3 standard plasmid

Release factor 2 (RF2) is a protein that causes termination of translation by recognizing the stop codon in an mRNA sequence. This part was originally designed by Lethbridge iGEM team 2017(http://2017.igem.org/Team:Lethbridge) The original composite part can be found here: https://parts.igem.org/Part:BBa_K2443030 RF2 has been Codon optimized for use in Escherichia coli and contains a N-Terminal hexahistidine tag. It is under the regulation of T7 Promoter, RBS and double terminator.

We have improved the part by successfully inserting it into the pSB1C3 standard plasmid. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 659
Illegal BglII site found at 1116
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Release factor 2 i,proved with insertion into pSB1C3 standard plasmid
+Release factor 2 (RF2) is a protein that causes termination of translation by recognizing the stop codon in an mRNA sequence. This part was originally designed by Lethbridge iGEM team 2017(http://2017.igem.org/Team:Lethbridge) The original composite part can be found here: https://parts.igem.org/Part:BBa_K2443030 RF2 has been Codon optimized for use in Escherichia coli and contains a N-Terminal hexahistidine tag. It is under the regulation of T7 Promoter, RBS and double terminator.
+We have improved the part by successfully inserting it into the pSB1C3 standard plasmid.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===