Difference between revisions of "Part:BBa K2692002:Design"
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===Source=== | ===Source=== | ||
| − | This part was synthesized as a g-block | + | This part was synthesized as a g-block through IDT. |
===References=== | ===References=== | ||
[1] Olson N., Gingery, M., Eiserling, F., and Baker, T. (2001) The structure of isometric capsids of bacteriphage T4. Virology. 279,385-391 | [1] Olson N., Gingery, M., Eiserling, F., and Baker, T. (2001) The structure of isometric capsids of bacteriphage T4. Virology. 279,385-391 | ||
Revision as of 02:41, 11 October 2018
Major T4 Capsid Protein gp24
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 1577
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1578
Illegal PstI site found at 1592
Illegal NotI site found at 1585 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 753
Illegal BamHI site found at 1248 - 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 1578
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1578
Illegal PstI site found at 1592 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The addition of a his tag was considered for purification purposes
Source
This part was synthesized as a g-block through IDT.
References
[1] Olson N., Gingery, M., Eiserling, F., and Baker, T. (2001) The structure of isometric capsids of bacteriphage T4. Virology. 279,385-391