Difference between revisions of "Part:BBa K2692001:Design"

 
(→‎Source)
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
The part was synthesized into a g-block
+
The part was synthesized as a g-block (linear Piece of DNA) through IDT.
  
 
===References===
 
===References===

Revision as of 02:15, 11 October 2018


Major T4 Capsid Protein gp23


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
    Illegal NotI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal BamHI site found at 1069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1118


Design Notes

The addition of a his tag for purification purposes


Source

The part was synthesized as a g-block (linear Piece of DNA) through IDT.

References