Difference between revisions of "Part:BBa K2633006:Experience"

Revision as of 01:00, 11 October 2018

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Applications of BBa_K2633006

This part was transformed using a restriction site illegal version of BBa_K2633000[1]. It was then used to inoculate Brachypodium distachyon seeds. Transformed bacteria expressing GFP were observed on and inside roots from 1 to 4 weeks post inoculation.

Figure 1. A) FCP2-01 transformed with GFP (left) vs wild-type (right) under blue light with orange filter. B) Transformed colonies on an agar plate.

Figure 2.[2] 3 weeks post inoculation on plant roots. A) FCP2-01 forming large colonies abount 2 mm long in B. distachyon roots B) Detail of panel A. C) FCP2-01 growing between plant cells on the root surface. D) Detail of colonies on root epidermis.

T--MichiganState--root_colonization_3wk_GA_results_small.png

Figure 3.[3] 4 weeks post inoculation on plant roots. A) FCP2-01 on B. distachyon roots. B) FCP2-0 colonizing root hairs. C) FCP2-01 growing on epidermis of the root. D) Enlarged image of panel C.

T--MichiganState--root_colonization_4wk_GA_results_small.png

User Reviews

UNIQf9edea1828472c28-partinfo-00000000-QINU UNIQf9edea1828472c28-partinfo-00000001-QINU

@@ Line 11: / Line 11: @@
 https://static.igem.org/mediawiki/parts/d/db/T--MichiganState--transformation_results_small.png
-Figure 2. 3 weeks post inoculation on plant roots. A) FCP2-01 forming large colonies abount 2 mm long in ''B. distachyon'' roots
+Figure 2.[https://static.igem.org/mediawiki/parts/thumb/1/13/T--MichiganState--root_colonization_3wk_GA_results_full.png/900px-T--MichiganState--root_colonization_3wk_GA_results_full.png] 3 weeks post inoculation on plant roots. A) FCP2-01 forming large colonies abount 2 mm long in ''B. distachyon'' roots
 B) Detail of panel A. C) FCP2-01 growing between plant cells on the root surface. D) Detail of colonies on root epidermis.
 https://static.igem.org/mediawiki/parts/6/66/T--MichiganState--root_colonization_3wk_GA_results_small.png
-Figure 3. 4 weeks post inoculation on plant roots. A) FCP2-01 on ''B. distachyon'' roots. B) FCP2-0 colonizing root hairs.
+Figure 3.[https://static.igem.org/mediawiki/parts/thumb/c/c4/T--MichiganState--root_colonization_4wk_GA_results_full.png/900px-T--MichiganState--root_colonization_4wk_GA_results_full.png] 4 weeks post inoculation on plant roots. A) FCP2-01 on ''B. distachyon'' roots. B) FCP2-0 colonizing root hairs.
 C) FCP2-01 growing on epidermis of the root. D) Enlarged image of panel C.