Difference between revisions of "Part:BBa K2617006"

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==Characterization==
 
==Characterization==
  
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During the production process, the accumulation of butanol will exert a great inhibitory effect on cell growth. Therefore, we will introduce the GroEL gene and GroES gene from clostridium acetone butanol to make escherichia coli heterologous expression of heat shock protein -- a molecular chaperone, which has been shown to increase butanol production [1].To test butanol tolerance, the control strain DH5α, and the GroESL overexpression strain DH5α were grown in the presence of butanol in concentrations up to 1%.  
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During the production process, the accumulation of butanol will exert a great inhibitory effect on cell growth. Therefore, we will introduce the GroEL gene and GroES gene from clostridium acetone butanol to make escherichia coli heterologous expression of heat shock protein (a molecular chaperone), which has been shown to increase butanol production [1].To test butanol tolerance, the control strain DH5α, and the GroESL overexpression strain DH5α were grown in the presence of butanol in concentrations up to 1%.  
  
 
[[File:T--UESTC-China--GroESL in DH5α.png|500px|thumb|center|'''Fig. 1''' The measurement of optical density (A600) by periodically taking samples.]]
 
[[File:T--UESTC-China--GroESL in DH5α.png|500px|thumb|center|'''Fig. 1''' The measurement of optical density (A600) by periodically taking samples.]]

Revision as of 13:34, 10 October 2018


GroEL

Promoting folding of misfolded polypeptides and enhancing the tolerance of Escherichia coli to butanol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 880
    Illegal AgeI site found at 1341
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

During the production process, the accumulation of butanol will exert a great inhibitory effect on cell growth. Therefore, we will introduce the GroEL gene and GroES gene from clostridium acetone butanol to make escherichia coli heterologous expression of heat shock protein (a molecular chaperone), which has been shown to increase butanol production [1].To test butanol tolerance, the control strain DH5α, and the GroESL overexpression strain DH5α were grown in the presence of butanol in concentrations up to 1%.

Fig. 1 The measurement of optical density (A600) by periodically taking samples.

References

1.Zingaro, K. A., & Papoutsakis, E. T. (2013). GroESL overexpression imparts Escherichia coli tolerance to i-, n-, and 2-butanol, 1, 2, 4-butanetriol and ethanol with complex and unpredictable patterns. Metabolic engineering, 15, 196-205.