Difference between revisions of "Part:BBa K2617006"
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[[File:T--UESTC-China--GroESL in DH5α.png|500px|thumb|center|'''Fig. 1''' The measurement of optical density (A600) by periodically taking samples.]] | [[File:T--UESTC-China--GroESL in DH5α.png|500px|thumb|center|'''Fig. 1''' The measurement of optical density (A600) by periodically taking samples.]] | ||
| + | ===References=== | ||
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| + | 1.Zingaro, K. A., & Papoutsakis, E. T. (2013). GroESL overexpression imparts Escherichia coli tolerance to i-, n-, and 2-butanol, 1, 2, 4-butanetriol and ethanol with complex and unpredictable patterns. Metabolic engineering, 15, 196-205. | ||
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Revision as of 13:31, 10 October 2018
GroEL
Promoting folding of misfolded polypeptides and enhancing the tolerance of Escherichia coli to butanol.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 880
Illegal AgeI site found at 1341 - 1000COMPATIBLE WITH RFC[1000]
Characterization
During the production process, the accumulation of butanol will exert a great inhibitory effect on cell growth. Therefore, we will introduce the GroEL gene and GroES gene from clostridium acetone butanol to make escherichia coli heterologous expression of heat shock protein -- a molecular chaperone, which has been shown to increase butanol production [1].To test butanol tolerance, the control strain DH5α, and the GroESL overexpression strain DH5α were grown in the presence of butanol in concentrations up to 1%.
References
1.Zingaro, K. A., & Papoutsakis, E. T. (2013). GroESL overexpression imparts Escherichia coli tolerance to i-, n-, and 2-butanol, 1, 2, 4-butanetriol and ethanol with complex and unpredictable patterns. Metabolic engineering, 15, 196-205.