Difference between revisions of "Part:BBa K2557002"

 
Line 5: Line 5:
 
We added Bxb1 recombination sites, attB and attP, at both ends of the reporter gene RFP (Inverted). When Bxb1 is expressed, attB and attP are recognized, and the sequence of RFP is inverted to enable normal expression.
 
We added Bxb1 recombination sites, attB and attP, at both ends of the reporter gene RFP (Inverted). When Bxb1 is expressed, attB and attP are recognized, and the sequence of RFP is inverted to enable normal expression.
  
−
<!-- Add more about the biology of this part here
+
 
 
===Usage and Biology===
 
===Usage and Biology===
  
Line 13: Line 13:
  
  
−
<!-- Uncomment this to enable Functional Parameter display
+
 
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2557002 parameters</partinfo>
 
<partinfo>BBa_K2557002 parameters</partinfo>
−
<!-- -->
 

Revision as of 12:59, 10 October 2018


Bxb1 attB-RFP-Bxb1 attP

We added Bxb1 recombination sites, attB and attP, at both ends of the reporter gene RFP (Inverted). When Bxb1 is expressed, attB and attP are recognized, and the sequence of RFP is inverted to enable normal expression.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 837
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 36
    Illegal BsaI site found at 177
    Illegal BsaI.rc site found at 885
    Illegal SapI site found at 794


Functional Parameters