Difference between revisions of "Part:BBa K2549026"

(→‎=interaction between ZF21.16-VP64 and 8*ZF 21.16-minCMV promotor)
Line 3: Line 3:
 
<partinfo>BBa_K2549026 short</partinfo>
 
<partinfo>BBa_K2549026 short</partinfo>
  
−
This part is one of the response elements of our amplifier, also executing the combiner function. 8×ZF21.16 binding sites is assembled using two 4×ZF21.16 binding sites ([[Part:BBa_K2446008]]) with a biobrick scar between them. Minimal CMV ([[Part:BBa_K2549049]]) is a promotor providing very low basal expression and high maximal expression after induction. This part can switch on the expression of gene downstream after induced by our zinc finger-based transcription activator.
+
This part is one of the response elements of our amplifier, also executing the combiner function. 8*ZF21.16 binding sites is assembled using two 4*ZF21.16 binding sites ([[Part:BBa_K2446008]]) with a biobrick scar between them. Minimal CMV ([[Part:BBa_K2549049]]) is a promotor providing very low basal expression and high maximal expression after induction. This part can switch on the expression of gene downstream after induced by our zinc finger-based transcription activator.
  
 
<!-- Add more about the biology of this part here -->
 
<!-- Add more about the biology of this part here -->
Line 11: Line 11:
  
 
[[File:ZF2.jpg|none|540px|thumb|JJ Collins et al stated:''sTFs constructed from OPEN-engineered ZFs are orthogonal to one another. sTF43-8 activated noncognate Promoter21-16 due to the fortuitous creation of a sequence that is significantly similar to the binding sequence of 43-8, when the downstream BamHI restriction site is considered.'']]
 
[[File:ZF2.jpg|none|540px|thumb|JJ Collins et al stated:''sTFs constructed from OPEN-engineered ZFs are orthogonal to one another. sTF43-8 activated noncognate Promoter21-16 due to the fortuitous creation of a sequence that is significantly similar to the binding sequence of 43-8, when the downstream BamHI restriction site is considered.'']]
−
====interaction between ZF21.16-VP64 and 8*ZF 21.16-minCMV promotor====
+
====interaction between ZF21.16-VP64 and 8*ZF21.16-minCMV promotor====
  
 
<!-- -->
 
<!-- -->

Revision as of 12:57, 10 October 2018


8*ZF21.16-minCMV

This part is one of the response elements of our amplifier, also executing the combiner function. 8*ZF21.16 binding sites is assembled using two 4*ZF21.16 binding sites (Part:BBa_K2446008) with a biobrick scar between them. Minimal CMV (Part:BBa_K2549049) is a promotor providing very low basal expression and high maximal expression after induction. This part can switch on the expression of gene downstream after induced by our zinc finger-based transcription activator.

Biology

synthetic promotor operators regulated by artificial zinc finger-based transcription factors

JJ Collins et al have reported several synthetic promotor operators which can interact with artificial zinc finger-based transcription factors with high specificity and high orthogonality[1].

JJ Collins et al stated:sTFs constructed from OPEN-engineered ZFs are orthogonal to one another. sTF43-8 activated noncognate Promoter21-16 due to the fortuitous creation of a sequence that is significantly similar to the binding sequence of 43-8, when the downstream BamHI restriction site is considered.

interaction between ZF21.16-VP64 and 8*ZF21.16-minCMV promotor

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. ↑ A synthetic biology framework for programming eukaryotic transcription functions. Khalil AS, Lu TK, Bashor CJ, ..., Joung JK, Collins JJ. Cell, 2012 Aug;150(3):647-58 PMID: 22863014; DOI: 10.1016/j.cell.2012.05.045