Difference between revisions of "Part:BBa K2610044"
 
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<partinfo>BBa_K2610044 short</partinfo>
 
<partinfo>BBa_K2610044 short</partinfo>
  
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This composite part features the regulatory part promoter SoxS (BBa_K2610030) coupled to fluorescent protein mCherry (BBa_K180008) as well as the regulatory part promoter MicF (BBa_K2610007) coupled to GFP (BBa_E0040). It can be used to visualize upregulation of SoxS and KatG. The two reporter proteins were selected in such a manner that the fluorophore spectra would not overlap.
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This composite part features the regulatory part promoter SoxS (BBa_K2610030) coupled to fluorescent protein mCherry (BBa_J06504) as well as the regulatory part promoter MicF (BBa_K2610007) coupled to GFP (BBa_E0040). It can be used to visualize upregulation of SoxS and KatG. The two reporter proteins were selected in such a manner that the fluorophore spectra would not overlap.
  
 
The SoxR protein is activated in response to specific types of oxidative stress and acts in the SoxS/SoxR complex. MicF expression is stimulated by many factors such as oxidative stress, osmolarity increase and temperature increase.  
 
The SoxR protein is activated in response to specific types of oxidative stress and acts in the SoxS/SoxR complex. MicF expression is stimulated by many factors such as oxidative stress, osmolarity increase and temperature increase.  

Latest revision as of 12:49, 10 October 2018


pSoxS-mCherry-pMicF-GFP

This composite part features the regulatory part promoter SoxS (BBa_K2610030) coupled to fluorescent protein mCherry (BBa_J06504) as well as the regulatory part promoter MicF (BBa_K2610007) coupled to GFP (BBa_E0040). It can be used to visualize upregulation of SoxS and KatG. The two reporter proteins were selected in such a manner that the fluorophore spectra would not overlap.

The SoxR protein is activated in response to specific types of oxidative stress and acts in the SoxS/SoxR complex. MicF expression is stimulated by many factors such as oxidative stress, osmolarity increase and temperature increase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1976