Difference between revisions of "Part:BBa K2610043"

Latest revision as of 12:47, 10 October 2018

pSoxS-mCherry-pKatG-GFP

This composite part features the regulatory part promoter SoxS (BBa_K2610030) coupled to fluorescent protein mCherry (BBa_J06504) as well as the regulatory part promoter KatG (BBa_K2610004) coupled to GFP (BBa_E0040). It can be used to visualize upregulation of SoxS and KatG. The two reporter proteins were selected in such a manner that the fluorophore spectra would not overlap.

The SoxR protein is activated in response to specific types of oxidative stress and acts in the SoxS/SoxR complex. The KatG gene encodes for hydroperoxidase I. It protects aerobic, phosphate-starved cells from oxidative damage. The expression of KatG is activated by the global regulator OxyR.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1075
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1818

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2610043 short</partinfo>
-This composite part features the regulatory part promoter SoxS (BBa_K2610030) coupled to  fluorescent protein mCherry (BBa_K180008) as well as the regulatory part promoter KatG (BBa_K2610004) coupled to GFP (BBa_E0040). It can be used to visualize upregulation of SoxS and KatG. The two reporter proteins were selected in such a manner that the fluorophore spectra would not overlap.
+This composite part features the regulatory part promoter SoxS (BBa_K2610030) coupled to  fluorescent protein mCherry (BBa_J06504) as well as the regulatory part promoter KatG (BBa_K2610004) coupled to GFP (BBa_E0040). It can be used to visualize upregulation of SoxS and KatG. The two reporter proteins were selected in such a manner that the fluorophore spectra would not overlap.
 The SoxR protein is activated in response to specific types of oxidative stress and acts in the SoxS/SoxR complex. The KatG gene encodes for hydroperoxidase I. It protects aerobic, phosphate-starved cells from oxidative damage. The expression of KatG is activated by the global regulator OxyR.