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=== III. Design Considerations === | === III. Design Considerations === | ||
Revision as of 12:23, 10 October 2018
Contents
LL-37 with L.jensenii specific strong promoter RpsU
I. Part BBa K2666001 : Function
The Montpellier iGEM team 2018 designed a construction that produce RFP in Bacillus subtilis (http://2018.igem.org/Team:Montpellier/Toolbox). For that, we used the promoter R0, a B.subtilis specific strong promoter [1]. Figure 1 illustrates the detailed design.
Figure 1 : Construct design. The sequence that encode RFP fusionned with a strong promoter R0.
The objective is to check the strength of several construction in E.coli, L.jensenii & B.subtilis.
II. Proof of function
III. Design Considerations
We added spacers to all of our constructions to unable easier use of the sequence and separation of the different genes of the sequences. We used two Terminators to our sequences :BBa_B0014 & BBa_B0015 to ensure the stopping of the transcription.
Reference:
[1] Guiziou, Sarah, et al. "A part toolbox to tune genetic expression in Bacillus subtilis." Nucleic acids research 44.15 (2016): 7495-7508.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 62
Illegal BamHI site found at 194 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]