Difference between revisions of "Part:BBa K2610042"
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Promoter GapA is naturally occurring constitutive promoter in Escherichia coli. | Promoter GapA is naturally occurring constitutive promoter in Escherichia coli. | ||
| − | Usage | + | ===Usage and Biology=== |
We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter (BBa_K2610040) from Escherichia coli with the link chromoprotein AsPink (BBa_K1033927) in order for us to compare it to our novel stress promoter+chromoprotein constructs. GapA is known for its high constitutive expression, as can be seen in Figure 1. | We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter (BBa_K2610040) from Escherichia coli with the link chromoprotein AsPink (BBa_K1033927) in order for us to compare it to our novel stress promoter+chromoprotein constructs. GapA is known for its high constitutive expression, as can be seen in Figure 1. | ||
Revision as of 12:00, 10 October 2018
pGapA-spisPink
Promoter GapA is naturally occurring constitutive promoter in Escherichia coli.
Usage and Biology
We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter (BBa_K2610040) from Escherichia coli with the link chromoprotein AsPink (BBa_K1033927) in order for us to compare it to our novel stress promoter+chromoprotein constructs. GapA is known for its high constitutive expression, as can be seen in Figure 1.
Figure 1. Constitutively expressed AsPink
References
Munjal, N., Jawed, K., Wajid, S., & Yazdani, S. S. (2015). A constitutive expression system for cellulase secretion in Escherichia coli and its use in bioethanol production. PloS one, 10(3), e0119917.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 140