Difference between revisions of "Part:BBa K2610041"
(→Usage and Biology) |
|||
| Line 6: | Line 6: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter from Escherichia coli with the blue chromoprotein AmilCP (BBa_K592009) in order for us to compare it to our novel stress promoter+chromoprotein constructs. GapA is known for its high constitutive expression, as is demonstrated in Figure 1. | + | We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter (BBa_K2610040) from Escherichia coli with the blue chromoprotein AmilCP (BBa_K592009) in order for us to compare it to our novel stress promoter+chromoprotein constructs. GapA is known for its high constitutive expression, as is demonstrated in Figure 1. |
Moreover, GapA being a natural occurring promoter there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors. | Moreover, GapA being a natural occurring promoter there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors. | ||
Revision as of 11:51, 10 October 2018
pGapA-AmilCP
Promoter GapA is naturally occurring constitutive promoter in Escherichia coli.
Usage and Biology
We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter (BBa_K2610040) from Escherichia coli with the blue chromoprotein AmilCP (BBa_K592009) in order for us to compare it to our novel stress promoter+chromoprotein constructs. GapA is known for its high constitutive expression, as is demonstrated in Figure 1.
Moreover, GapA being a natural occurring promoter there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors.
Figure 1. Constitutively expressed AmilCP
References
Munjal, N., Jawed, K., Wajid, S., & Yazdani, S. S. (2015). A constitutive expression system for cellulase secretion in Escherichia coli and its use in bioethanol production. PloS one, 10(3), e0119917.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 140