Difference between revisions of "Part:BBa K2556032"

 
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__NOTOC__
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<partinfo>BBa_K2556032 short</partinfo>
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The lac repressor controlled by arabinose binds to the lac operator to inhibit transcription.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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This part is mainly composed of three elements:
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(1)Arabinose induced promoter PBAD to express the gene of downstream.This part contains the promoter as well as the coding sequence for the repressor AraC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, AraC changes its conformation. This causes the protein to diffuses from the DNA thereby inducing transcription.
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(2)This part contains the lac operator as well as the coding sequence for the repressor LacI.The LacI repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D- thiogalactopyranoside (IPTG).
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(3)GFP, as the report gene, can verify the degree of repression by LacI repressor. The lower fluorescence，the higher degree of repression.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2556032 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2556032 parameters</partinfo>
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Revision as of 11:33, 10 October 2018


The lac repressor controled by arabinose binds to the lac operator to inhibit transcription

The lac repressor controlled by arabinose binds to the lac operator to inhibit transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2640
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3159
    Illegal XhoI site found at 3060
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961